A NEW MINIMALLY INVASIVE METHOD FOR MEASURING BLOOD PRESSURE IN THE CAUDAL VENTRAL ARTERY IN RATS — EVALUATION

Abstract

Introduction. Blood pressure is an important hemodynamic indicator characterizing the state of the  cardiovascular system, which plays an important role in the experimental modeling of pathological conditions associated with impaired hemodynamics (for example, blood loss or hemorrhagic shock) in biological test systems. In modern practice, invasive and non-invasive blood pressure measurement methods are used in conducting research. However, if the objectives of the study include the registration of blood pressure in modeling pathological conditions associated with impaired hemodynamic processes, such as hemorrhagic shock, then with existing methods of measuring blood pressure, obtaining accurate, reliable data is often difficult. The aim of the study was to develop a minimally invasive, continuous method for recording blood pressure in the caudal artery in rats, both under anesthesia and without it, including without the use of anticoagulants; comparative analysis of various blood pressure measurement techniques in intact animals, in experimental modeling of hemorrhagic shock. Materials and methods. 110 male rats, Sprague Dawley, weighing 360±20 g were involved in the work. During the experiment, a minimally invasive method of blood pressure registration was developed, surgical modeling of hemorrhagic shock was performed, and a comparative analysis of various blood pressure measurement methods was carried out. The results of the study. It was found that the developed method can be considered accurate and low-traumatic, allowing fast, continuous blood pressure measurements in real time, both under general anesthesia and without it, without the use of anticoagulants. The accuracy of measurements does not depend on the nature, complexity of the experimental study and the general hemodynamic picture of the animal.