STUDY OF LYMPHOCYTES POPULATION BY METHOD OF FLOW CYTOMETRY IN PATIENTS WITH PRE-OPERATIVE NUTRITIVE SUPPORT WITH SURGICAL INTERVENTION ON ORGANS OF THE GASTROINTESTINAL TRACT

  • В Э Дорджиев Saint-Petersburg State Pediatric Medical University, 194100, Saint-Petersburg, Litovskaya str., 2
  • К Ю Красносельский Saint-Petersburg State Pediatric Medical University, 194100, Saint-Petersburg, Litovskaya str., 2
  • Ю С Александрович Saint-Petersburg State Pediatric Medical University, 194100, Saint-Petersburg, Litovskaya str., 2
  • Е А Дементьева Saint-Petersburg State Pediatric Medical University, 194100, Saint-Petersburg, Litovskaya str., 2
  • О П Гурина Saint-Petersburg State Pediatric Medical University, 194100, Saint-Petersburg, Litovskaya str., 2
Keywords: lymphocyte population, flow cytometry, nutritional support, surgery, immune compounds, omega-3 fatty acids, L-carnitine, L-glutamine and BCAA

Abstract

The study included 12 patients aged 49 to 76 years, who routinely performed the surgical
intervention on the organs of abdominal cavity. The patients were divided into two groups. In the first
(main) group (6 persons), in addition to a standard surgical table for nutritional support used Impact-oral
(Nestle), omega–3 fatty acids, L-carnitine, L-glutamine and BCAA. The control group has not received
nutritional support. Immunophenotyping of lymphocytes was performed by flow cytometry using direct
immunofluorescence assay of whole peripheral blood and “’no clean ‘” technology (flow cytometer Epics
XL,Coulter Vismap, USA). There is stability in the population Of t-lymphocytes in the postoperative
period in the main group.

Published
2019-03-14
How to Cite
Дорджиев, В. Э., Красносельский, К. Ю., Александрович, Ю. С., Дементьева, Е. А., & Гурина, О. П. (2019). STUDY OF LYMPHOCYTES POPULATION BY METHOD OF FLOW CYTOMETRY IN PATIENTS WITH PRE-OPERATIVE NUTRITIVE SUPPORT WITH SURGICAL INTERVENTION ON ORGANS OF THE GASTROINTESTINAL TRACT. Medicine: Theory and Practice, 3(3 приложен), 153-156. Retrieved from https://ojs3.gpmu.org/index.php/med-theory-and-practice/article/view/363
Issue
Vol 3 No 3 приложен (2018)
Section
Статьи