ENDOTHELIAL DYSFUNCTION IN THE PATHOGENESIS OF NONALCOHOLIC FATTY LIVER DISEASE IN RATS, AND METHODS FOR ITS CORRECTION

Abstract

Analysis of structural and functional features of endothelium of blood vessels in rats with fatty degeneration of the liver of varying severity in case of correction with succinyladenosine drug Remaxol was performed in a model of fruktoze-induced non-alcoholic fatty liver disease of varying severity in laboratory rats: semi - light severity of the disease (non-alcoholic hepatic steatosis) and average degree of severity of the disease (nonalcoholic steatohepatitis). The liver lesion were modeled by adding fructose to food (60%) resulting in steatohepatitis, or drinking water (10%) - steatosis of the liver. The models were characterized by activation of the hemostatic system of experimental animals. The severity of metabolic disturbances of the rats depended on the severity of the simulated disease. For analysis of endothelial dysfunction in experimental groups concentrations of vascular endothelial growth factor, nitric oxide II, endothelin-1, circulating endothelial cells were determined. The results characterizing the level of lethality in experimental groups confirm the adequacy of the choice of the experimental models of liver damage. Application Remaxol for the treatment of fructose-induced fatty liver distrophy produced a pronounced therapeutic effect, which was evident in a statistically significant reduction in mortality. The ongoing 10-day therapy with Remaxol caused some normalization of structural and functional state of endotheliocytes of experimental animals.