INFORMATIVE VALUE OF KEY MOLECULAR AND HISTOLOGICAL MARKERS IN THE MODELING OF BRONCHOPULMONARY DYSPLASIA

Abstract

Advances in modern medicine make it possible to nurse very premature babies, but, due to vital mechanical ventilation, the development of a “new” bronchopulmonary dysplasia is often observed. BPD is still an urgent problem in pediatrics, it is necessary to develop approaches to modeling this problem for a more thorough study of the pathogenesis of diseases and the search for new effective methods of therapy. The aim of our study is to show the possibility of modeling bronchopulmonary dysplasia in rats using 14-day hyperoxia. During this period, the experimental group of newborn animals was kept in a sealed chamber with a constant supply of 80 % oxygen, the control group under normal conditions. After this time, an animal weight measurement, morphometry of the lung tissue, expression of tnf-a, vegf were carried out. The study protocol was approved by the commission for the control of the maintenance and use of laboratory animals Almazov NMRC. As a result of the experiment, the experimental group showed a significant reduction in animals weight, simplification of alveolarization, pro-inflammatory inflammation of the lung tissue and a decrease in vasculogenesis, which corresponds to the «new» BLD phenotype. Therefore, the use of this rat model of hyperoxia-induced BPD may fulfill the need for experimental models used in research of the BPD pathogenesis and evaluation of potential therapeutic agents.