BIOLOGICAL CHARACTERISTICS OF THE BIODEGRADABLE MATERIAL FOR BONE REPAIR

Abstract

The biological properties of the biodegradable bone replacement material obtained from three main components: Bombyx mori silk fibroin, calcium phosphates and casein were evaluated ex vivo, the tests consisted of a 24-hour cytotoxicity study using a culture of mononuclear leukocytes from a healthy dog and using human leukemic T lymphoblast like cells. In the culture of multipotent mesenchymal stromal cells (MMSCs) of dog adipose tissue, the osteogenic potential of the samples was determined by measuring the secretion of osteocalcin, determining the mineralization of the intercellular matrix (alizarin staining). Analysis of the results of the study showed more pronounced ex vivo processes of mineralization of the intercellular substance with the highest percentage of calcium phosphates in the material. With 24-hour in vitro contact with samples of material of three different compositions, the death rate (mainly by necrosis) of leukemia T lymphoblast like cells increased by 5-7 %, with the highest calcium phosphate content, while the content of mononuclear leukocytes of a healthy dog was not reflected.