DEPENDENCE OF CELLULAR IMMUNITY ON THE INDEX OF ANABOLISM AT ATHLETES WITH THE ARRHYTHMIC FORM OF THE CARDIOMYOPATHY OF THE OVERSTRAIN

Abstract

For the purpose of identification of overtraining condition and its connection to arrhythmic variant of stress cardiomyopathy and dysimmunity we have studied relative numbers and functional activity of immunocompetent lymphocytes in the blood of oarsmen depending on testosterone-hydrocortisone ratio with arrhythmic variant of stress cardiomyopathy. 62 male oarsmen athletes were included in the study, which lasted one annual training cycle. According to the result of the electrocardiogram, we have analyzed an atrioventricular complex and heart rate. Blood hydrocortisone and testosterone concentration was determined using Boehringer Mannheim Immunodiagnostics ES 30 analyzer. Anabolic index was calculated for testosterone/cortisol ratio. Blood T-lymphocytes (CD3 + cells), T-helpers (CD4 + cells), cytotoxic T-cells (CD8 + cells), lymphocytes with activation phenotype (CD25+, CD95+) was determined in microlymphocytotoxic test modified by A. Iskhakov. According to the results of the studies performed, the secondary immunodeficiency was identified in sportsmen with arrhythmic variant of stress cardiomyopathy mainly at decrease of the anabolic index. In the absence of anabolic index abnormalities in case of arrhythmic variant of stress cardiomyopathy, by contrast an activation of immune processes was identified. So in case of arrhythmic variant of stress cardiomyopathy when anabolic processes in the organism are normal, activation of immune system results in increase in relative number of lymphocytes with activation phenotype of expressing differentiation activation antigens (cells CD95+ and CD25+) and in increase of spontaneous proliferative activity of T - and B-lymphocytes. In arrhythmic variant of stress cardiomyopathy in parallel with decrease in anabolic index below 3% the sportsmen show a decrease in the relative number of killer lymphocytes (CD16+) and due to increase in the number of cytotoxic lymphocytes (CD8) there is statistically significant decrease in the immune regulation index. Disorders of immunity B-system are a decrease in both relative number of B-lymphocytes and their proliferative potential in the absence of any increase of spontaneous proliferative activity.