EVALUATION OF THE GENUS CANDIDA FUNGI ADHESIVE PROPERTIES ON THE MATERIALS USED IN DENTISTRY

  • A.V. Zachinyaeva Saint Petersburg State Pediatric Medical University. Lithuania 2, Saint Petersburg, Russian Federation, 194100 https://orcid.org/0000-0003-1521-3060
  • Ya.V. Zachinyaev Petrozavodsk State University. Lenin str. 33, Petrozavodsk, Russian Federation, Republic of Karelia, 185910
  • D.P. Gladin Saint Petersburg State Pediatric Medical University. Lithuania 2, Saint Petersburg, Russian Federation, 194100
  • I.A. Baranov North-Western Medical University named after I.I. Mechnikov. Kirochnaya 41, Saint Petersburg, Russian Federation, 191015
  • A.S. Nabieva Saint Petersburg State Pediatric Medical University. Lithuania 2, Saint Petersburg, Russian Federation, 194100
  • O.G. Gorbunov Saint Petersburg State Pediatric Medical University. Lithuania 2, Saint Petersburg, Russian Federation, 194100
  • A.N. Andreeva Saint Petersburg State Pediatric Medical University. Lithuania 2, Saint Petersburg, Russian Federation, 194100
Keywords: fungi of the genus Candida, Candida albicans, biofilms, acrylamide plastics

Abstract

Introduction. Acrylamide plastics are widely used in orthopedic dentistry. Studies of their susceptibility to microbial adhesion are relevant, since the restorative material made on the basis of these polymers can become a reservoir for microorganisms that can infect peri-implant tissues and cause inflammation. The purpose of the study was to test the adhesive ability of clinical strains of Candida to samples of acrylamide plastics. Materials and methods. 50 clinical strains of Candida fungi have been studied for the formation of biofilms during cultivation on acrylamide plastics. Plastic samples were treated with an inoculum of fungal cultures for 48 h at 37 °C. The values of their optical density (λ=560 nm) were a quantitative assessment of the biomass of the formed films. The results of the study. Quantitative analysis of the biofilm biomass showed that after 48 h all fungal strains formed a biofilm on the surface of the tested polymer discs. The highest quantitative values of the biofilm biomass were noted in the cultivation of C. albicans. Conclusion. It was noted that the type of material is not a key growth restriction factor for C. albicans. A set of measures is needed that combines optimal mechanical processing methods with the use of antimicrobial drugs to prevent the formation and accumulation of biofilms.

References

Антонова Е.А., Косякова К.Г. Грибы рода Candida — возбудители инфекционных состояний. Здоровье — основа человеческого потенциала. 2019; 14(1): 455–9.

Зачиняева А.В., Зачиняев Я.В. Действие полигексаметиленгуанидина на активность каталазы гриба Pyricularia oryzae. Успехи медицинской микологии. 2022; 23: 245–6.

Козлова Н.С., Баранцевич Н.Е., Косякова К.Г. и др. Чувствительность к антибиотикам энтеробактерий, выделенных в двух стационарах двух районов Санкт-Петербурга. Проблемы медицинской микологии. 2017; 19(1): 34–42.

Козлова Н.С., Нестерова Е.В., Васильев О.Д., Селезнева А.А. Спектры устойчивости к антифунгальным препаратам штаммов Candida spp., выделенных в кожно-венерологическом диспансере. Успехи медицинской микологии. 2023; 24: 221–5.

Козлова Н.С., Нестерова Е.В., Трофимова Н.Н. и др. Антибиотикорезистентность штаммов Сandida spp., выделенных у больных дерматозами. Здоровье — основа человеческого потенциала: проблемы и пути их решения. 2021; 16(2): 429–35.

Кузьмина Д.А., Новикова В.П., Шабашова Н.В., Оришак Е.А. Candida spp. и микробоценоз полости рта у детей с декомпенсированной формой кариеса. Проблемы медицинской микологии. 2011; 13(1): 23–7.

Bertolini M., Costa R.C., Barão VAR. Oral Microorganisms and Biofilms: New Insights to Defeat the Main Etiologic Factor of Oral Diseases. Microorganisms. 2022; 10(12): 2413.

Cássia M. de Souza, Murilo M. dos Santos, Luciana Furlaneto-Maia. Adhesion and biofilm formation by the opportunistic pathogen Candida tropicalis: what do we know? Canadian Journal of Microbiology. 2023; 69(6): 207–18.

Engel A.S., Kranz H.T., Schneider M. et al. Biofilm formation on different dental restorative materials in the oral cavity. BMC Oral Health. 2020; 20 (1): 162.

Kuhn D.M., Chandra J., Mukherjee P.K. Comparison of Biofilms Formed by Candida albicans and Candida parapsilosis on Bioprosthetic Surfaces. Infect Immun. 2002; 70(2): 878–88.

Mazurek-Popczyk J., Nowicki A., Arkusz K. et al. Valuation of biofilm for-mation on acrylic resins used to fabricate dental temporary restorations with the use of 3D printing technology. BMC Oral Health. 2022; 22(1): 442.

Paulone S., Malavasi G., Ardizzoni A. Candida albicans survival, growth and biofilm formation are differently affected by mouthwashes: an in vitro study. New Microbiologica. 2017; 40(1): 45–52.

Published
2024-05-08
How to Cite
Zachinyaeva, A., Zachinyaev, Y., Gladin, D., Baranov, I., Nabieva, A., Gorbunov, O., & Andreeva, A. (2024). EVALUATION OF THE GENUS CANDIDA FUNGI ADHESIVE PROPERTIES ON THE MATERIALS USED IN DENTISTRY. Russian Biomedical Research, 8(4), 27-31. https://doi.org/10.56871/RBR.2023.97.69.004
Section
Статьи