IMMUNOHISTOCHEMICAL PICTURE OF ADHESIONS IN THE ABDOMINAL CAVITY IN CHILDREN WITH NON - DIFFERENTIATED CONNECTIVE TISSUE DYSPLASIA

Abstract

A study of the immunohistochemical (IHC) picture of adhesions of the abdominal cavity with adhesive intestinal obstruction (SKN) in children with associated undifferentiated connective tissue dysplasia (NDST). Materials and methods. We studied the biomaterial of 16 children operated on for SKN. There were 10 boys, 6 girls. Age - 8.6 ± 1.4 years. Patients are divided into 2 groups: 1 group (9 patients without NDCT) and 2 group (7 children with associated NDCT). Material for the study was taken during laparoscopic or traditional adhesiolysis. IHC was carried out on serial paraffin sections with a thickness of 5 μm, placed on adhesive glasses coated with polylysine (Menzel-Glaser, Germany). Primary antibodies are monoclonal antibodies to type I, II, III and IV collagen (Dako, Denmark), to Laminin, to angiogenesis factors (VEGF) and fibroblast growth factor (bFGF). The amount of brown colored collagen fibers was determined in 10 fields of view over an area of 502.08 μm2 (magnification × 40; × 100; × 400 of the Olympus CX-41 microscope). Results. IHC in group 1 revealed a predominance of type I collagen with less collagen type II and type III. In group 2, the absence of type I collagen in commissures was noted. In this case, type II collagen is randomly located, and type III collagen is present in fibrils. The ratio of collagen type I to co II is 1.4: 3.9. Type IV collagen was absent. In group 1, there is a pronounced accumulation of bFGF in endotheliocytes and Laminin in the basement membrane, rather than VEGF. Conclusions: Thus, the different collagen content in adhesions in children with SKN, in children with and without NDCT, necessitates further study of this indicator as a predictor of the development of adhesions of the abdominal cavity.