TRANSGENERATIONAL EFFECT OF PRENATAL ACTION OF SYNESTROL ON MORPHOFUNCTIONAL CHARACTERISTICS OF OVARIES OF OFFSPRING OF LABORATORY MICE

Abstract

Scientific studies of the prenatal effect of estrogens indicate an increase in the hormonal background and the formation of morphofunctional changes in the reproductive organs of future offspring. The aim of the study was to study the transgenerational effect of prenatal action of synestrol on the morphofunctional characteristics of ovaries of laboratory mice offspring, with subsequent study of their morphometric and immunohistochemical parameters. Experimental animals were divided into 4 groups. Control group animals were injected once of olive oil at a dose of 0.2 mg/kg intramuscularly, the first experimental group C25 intramuscularly injected sinestrol in the dose of 25 µg/kg, in 2 % oil solution of sinistrol, the second experimental group C40 intramuscularly injected sinistrol at a dose of 40 mg/kg in 2 % oil solution of sinistrol, the third experimental group C50 - a single intramuscular sinistrol at a dose of 50 µg/kg, in 2 % oil solution of sinistrol, in the stage of gestation E 11.5. Histological study of ovarian preparations of the offspring of the first experimental group of C25 mice showed a change in the ratio of cortical matter to brain matter, a decrease in the number of follicles compared to the control group. As a result of intramuscular single administration of synestrol to the second experimental group C40, an increase in the areas of the brain and cortical substances in the ovary of the offspring was observed in comparison with the control group. Morphometric calculation of the number of ovarian follicles shows a decrease in their number, in contrast to the indicators of the control group. In the third experimental group C50 in the ovary of the offspring, there is a persistent morphological changes in the increase of the area of cortex and medulla, a significant increase in the area of yellow body, decrease of the number of all types of follicles. The effect of exposure to different doses of synestrol in the prenatal period should be considered as possible morphofunctional predictors of disorders of the reproductive system of offspring as a whole.