MODERN APPROACHES TO THE DETERMINATION OF PARAMETERS FOR THE EXCLUSION OF IMAGE ARTIFACTS IN DIGITAL MICROPHOTOGRAPHY © Artem

Abstract

Purpose of the research. Determination of artifact exclusion parameters on digital micrographs of lymphocytes. Materials and methods. Analysis of publications in the RSCI, Scopus; digital micrographs to assess the number of cells in the field of view. The study was conducted using the open source biomedical image analysis software QuPath. We used digital micrographs of lymphocytes with manual marking for cell counting, which are in the open databases openmicroscopy.org and openslide.org. For automatic calculation, linear characteristics were determined: the length of the core, the radius of curvature, angular parameters; areal characteristics: perimeter, area, orientation, projections on the X and Y axes, length of the maximum and minimum axes; shape coefficients: length, squareness, sphericity, roundness. Results. Digital microimages (n=69) of human lymphocytes were processed. Statistical processing of the results of manual, automatic and automatic counting with correction was carried out. Conclusions. Adjustment of the set of parameters gave a statistically significant result, allowing to filter out artifacts and take into account only cells.