EVALUATION OF THE EFFICACY OF TREATMENT OF 3RD DEGREE BURNS WITH HLDF6 PEPTIDE AND SILVER NANOPARTICLES IN CARBOPOL 2020 GEL IN VIVO EXPERIMENT
Abstract
Introduction. Between 250 and 280 thousand victims of deep thermal burns of the skin are registered annually in the territory of the Russian Federation; in every 6 cases the victims are underage patients. The lethality rate in case of thermal trauma is 7.2 %, but in case of extensive deep burns it reaches 13–14 %. Early surgical treatment of patients with severe thermal burns is an accepted standard and therefore the search for wound healing agents reducing the period of treatment and the risk of infectious complications is essential. Objective. To evaluate the efficacy of carbopol hydrogel with injected silver nanoparticles and HLDF6 peptide in the treatment of thermal burns of grade III skin in vivo. Materials and methods. The structure of the work is represented by an experimental study. The work was performed on 50 male Wistar rats, with an average weight of 230–250 g. The animals were divided into 5 groups depending on the applied concentration of HLDF6 peptide in Carbopol 2020 gel. Carbopol ETD 2020 hydrogel samples (0.5 %) containing 0,00015 % nano-silver and HLDF6 peptide with concentrations of 0 %, 0,01 %, 0,001 %, 0,0001 % and 0,00001 % were used to evaluate efficacy. A planimetric method was used to assess the dynamics of wound healing. The obtained data were subjected to statistical analysis using the Mann–Whitney U-parameter. Results. Application of 0,0001 and 0,00001 % doses of the peptide HLDF6 demonstrated activation of the healing processes on the 14th day of the experiment by 45,8 % and 31,7 % correspondingly (p < 0,01), and also reduced the incidence of purulent complications by 62,5 % (p < 0,05). The peptide concentration of 0,01 % shows an increase in the duration of treatment, and 0,001 %, no significant differences in comparison with the control and experimental groups. Conclusion. The use of low doses of the HLDF6 peptide (volume concentration in the range of 10–4–10–5 %) in gel preparations in the treatment of deep thermal burns of the skin shows high efficacy. Small concentrations of HLDF6 peptide allow significant activation of wound healing processes.
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